ROLE OF FRESH CARROT JUICE AGAINST CISPLATIN TOXICITY ON SPLEEN AND SOME BLOOD PARAMETERS IN RABBITS

Abstract

The current research targeted to study the role of the juice of the carrot against cisplatin toxicity in the spleen of male rabbits by using a single injection of cisplatin in an intraperitoneal of 5 mg/kg. The parameter counts of WBCs, PLT, LYM, MID, and GRA and the level of C3 complement protein were measured. The result showed changes in the hematological parameters in the group of CP treated and the group of CP treated with oral fresh carrot juice paralleled to the control group. WBCs and PLT count in serum samples show an important P- value < 0.05 decrease in the serum of rabbits treated with cisplatin paralleled to the negative control group. Moreover, there were decreases in LYM and MID counts but non-significant in the serum rabbits treated with CP paralleled to the group of negative control. However, the GRA count and level of C3 complement were increased in the serum rabbits treated with CP paralleled to the control negative group. After rabbits received an oral dose of fresh carrot juice 5ml/kg for the following 4 days before cisplatin injection, 3 following days after cisplatin injection, the level of C3 complement and counts of GRA were reduced in the group of CP-treated with oral fresh carrot juice, and succeeded in restoring the level of C3, count of GRA to the same range to the negative control. Moreover, the count of LYM slightly increased compared to the group of CP treated but not in the same range as the negative control. However, other parameters show no effect of the fresh carrot juice treatment.

Furthermore, for histopathological investigation, the spleen from all groups was collected. Histological observations of spleen show that fresh carrot juice has the role reduced the toxicity of cisplatin by reduce the effect of cisplatin in the tissues which reduce number of the white cells in red and with pulp. This study demonstrated that fresh carrot juice has a role as a potential therapeutic against (CP) toxicity in the immunity of rabbits.