PERFORMANCE COMPARISON OF BETWEEN CITRUS AURANTIFOLIA(LIME) SEED & MOMORDICA CHARANTIA (BITTER MELON) IN REDUCING BLOOD GLUCOSE

Abstract

Momordica Charantia (Bitter Melon) and Citrus Aurantifolia (Lime) seeds have been used as a traditional remedy in diabetics due to its hypoglycemic activity. However, its anti-hyperglycemic effect and anti-glycation activity has been demonstrated in vitro and for type-1 diabetics patient, but not in long term clinical study. The study aimed to develop plant based insulin from Momordica Charantia (Bitter Melon) and Citrus Aurantifolia (Lime) seeds in reducing blood glucose. To determine the functional group presence in the both plant based insulin, a qualitative study using Fourier-transform Infrared (FTIR) Spectroscopy was conducted. To compare the effectiveness of Citrus Aurantifolia (Lime) Seed and Momordica Charantia (Bitter Melon) compare with Insulin (Biphasic Isophane), an observation on blood glucose level based on patient’s dietary for a period of time had been carried out. Methods: Dried Citrus Aurantifolia (Lime) seeds and Momordica Charantia (Bitter Melon). Then, milled and soaked in ethanol for 3 days. Lime seed and bitter melon were filtered and evaporated to get extract product. Both product was run into FTIR to identify the qualitative of functional group. For patient diet, the patient had Citus Aurantifolia (Lime) seeds and Momordica Charantia (Bitter Melon) drink for 3 days continuously for each. Result: The functional group presence inside the Momordica Charantia (Bitter Melon) and Citrus Aurantifolia (Lime) seeds was 1,2,4-trisubstituted,Sulfoxide,Primary Alcohol, Phenol, Sulfonyl Chloride, Sulfate, Alkane, Alkene, Alcohol. Based on patient’s dietary, Momordica Charantia (Bitter Melon) shows the highest reduction on blood glucose level compared to Citus Aurantifolia (Lime) seeds and Insulin (Biphasic Isophane).